| Primary Identifier | MGI:6378994 | Allele Type | Endonuclease-mediated |
| Attribute String | Null/knockout | Gene | Zcchc8 |
| Strain of Origin | C57BL/6J | Is Recombinase | false |
| Is Wild Type | false |
| molecularNote | First, a single guide RNA (5'-TGAACATTCTGACAAGACCC-3') was designed to target sequences in exon 2. Next, a DNA oligo for homology directed repair (HDR) was designed to target a 33 bp insertion (BamHI site, Flag tag sequence and STOP sequence) into exon 2 that introduced a premature stop codon at amino acid 93 (P83delinsGSDYKDDDDK*). |
