| Primary Identifier | MGI:6364600 | Allele Type | Targeted |
| Attribute String | Constitutively active | Gene | Dicer1 |
| Transmission | Germline | Strain of Origin | 129S6/SvEvTac |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | This allele was generated from a vector that contains two TC>GA mutations (in exon 24 and 26) that create serine to aspartic acid phosphomimetic mutations that lead to a constitutively active enzyme: p.S1712D and p.S1836D. The vector has the potential to generate three different alleles (the two single mutations and one double mutation), depending on the location of the cross-over breakpoints during homologous recombination. The other allele that was created is Dicer1tm1.1Glo. The neomycin reistance gene cassette that was inserted into intron 24, was removed through subsequent cre-mediated recombination. |
