| Primary Identifier | MGI:6384879 | Allele Type | Endonuclease-mediated |
| Attribute String | Not Specified | Gene | Tnni3k |
| Strain of Origin | C57BL/6J | Is Recombinase | false |
| Is Wild Type | false |
| molecularNote | CRISPER/Cas9 genome editing is used to change the lysine codon at position 489 to arginine (AAA to AGA) in exon 15 of the gene. This mutation results in a premature stop codon homologous to one found in exon 16, early in the encoding portion of the kinase domain, in the naked mole-rat species Heterocephalus glaber. Two G-to-T substitutions were also introduced in order to eliminate the Cas9 PAM sequence to prevent retargeting. |
