| Primary Identifier | MGI:6385305 | Allele Type | Targeted |
| Attribute String | Recombinase | Gene | Robo3 |
| Transmission | Germline | Strain of Origin | B6(Cg)-Tyr<c-2J> |
| Is Recombinase | true | Is Wild Type | false |
| molecularNote | A targeting construct was generated by a combination of gene synthesis and recombineering. The Cre coding sequence and a frt-flanked neomycin (Neo) cassette were inserted into exon 1 of the Robo3 locus to replace the endogenous translation initiation codon and subsequent 31 nucleotides. The Neo cassette was subsequently deleted by crossing the chimeras to FLPe mice. |
