| Primary Identifier | MGI:6423080 | Allele Type | Targeted |
| Attribute String | Null/knockout, Reporter | Gene | Gpr27 |
| Transmission | Germline | Strain of Origin | 129S/SvEvBrd |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | The single coding exon was replaced with an IRES-lacZ-neomycin resistance cassette and a bovine growth hormone polyadenylation signal, followed by a PGK-puromycin resistance cassette. RT-qPCR analysis confirmed the absence of mRNA expression in the cerebral cortex and pancreatic islets isolated from homozygous mutant mice. Unexpectedly, Gpr27 deletion reduced expression of Eif4e3, a neighboring gene, likely by deleting transcription start sites on the anti-sense strand of the Gpr27 coding exon. |
