Primary Identifier | MGI:6402920 | Allele Type | Endonuclease-mediated |
Attribute String | Not Specified | Gene | Serpinb2 |
Strain of Origin | C57BL/6 | Is Recombinase | false |
Is Wild Type | false |
molecularNote | The active site (P1) Arg38020 (codon AGA) of Serpinb2 was changed to Ala380 (codon GCA); i.e. nucleotides 1222 and 1223 (with reference to accession NM_011111.4) were changed from AG to GC. Two silent mutations were also introduced to prevent cutting of the oligonucleotide during genome editing. |