Primary Identifier | MGI:6451773 | Allele Type | Targeted |
Attribute String | Null/knockout | Gene | Pld4 |
Transmission | Germline | Strain of Origin | 129 |
Is Recombinase | false | Is Wild Type | false |
molecularNote | A loxP site and an FRT site flanked neomycin resistance gene cassette were inserted into intron 3 and a second loxP site into intron 6. The neo cassette was removed through subsequent flp-mediated recombination and exons 4-6 were deleted through cre-mediated recombination. Immunohistochemistry showed no peptide is expressed from this allele. |