Primary Identifier | MGI:6503895 | Allele Type | Targeted |
Attribute String | Endonuclease | Gene | Gt(ROSA)26Sor |
Transmission | Germline | Strain of Origin | C57BL/6N |
Is Recombinase | false | Is Wild Type | false |
molecularNote | A human codon-optimized hemagglutinin-tagged Cas9 sequence derived from Streptococcus pyogenes was inserted into the Gt(ROSA)26Sor locus with a loxP-flanked puromycin resistance-containing stop cassette preceding the Cas9 sequence. This constitutively expressing allele was generated by deletion of the loxP-flanked stop cassette in the germline. |