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Allele : Stra8<em1Keish> stimulated by retinoic acid gene 8; endonuclease-mediated mutation 1, Kei-ichiro Ishiguro
Primary Identifier  MGI:6488093 Allele Type  Endonuclease-mediated
Attribute String  Epitope tag, Reporter Gene  Stra8
Strain of Origin  (C57BL/6J x 129S6/SvEvTac)F1 Is Recombinase  false
Is Wild Type  false
molecularNote  Sequence for three FLAG tags and an HA tag was inserted in-frame into the 3' UTR in exon 9, followed by p2A self-cleaving sequence and the GFP fluorescent marker gene, using CRISPR/Cas9 technology with gRNAs GCCTCAGGTCACATTATCGG(tgg) and TGCAATCAGTTCCGACTCTC(tgg). A neomycin resistance gene cassette was inserted downstream of the gene.
  • mutations:
  • Insertion
  • synonyms:
  • Stra8-3FH-p2A-GFP KI,
  • Stra8-3FH-p2A-GFP KI
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1 Feature

Genome

0 Expresses

0 Mutation Involves

Phenotype

Mouse alleles --> Mammalian phenotypes (MP terms)

 

Other

0 Carried By

0 Driven By

3 Publication categories





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