| Primary Identifier | MGI:6507948 | Allele Type | Targeted |
| Attribute String | Conditional ready | Gene | Fermt2 |
| Transmission | Germline | Strain of Origin | C57BL/6N |
| Is Recombinase | false | Is Wild Type | false |
| Project Collection | EUCOMM |
| molecularNote | The L1L2_gt1 cassette was inserted at position 45712791 of Chromosome 14 upstream of the critical exons 5-6 (Build GRCm39). The cassette is composed of an FRT flanked lacZ/neomycin sequence followed by a loxP site. An additional loxP site is inserted downstream of the targeted exons at position 45714360. The critical exons 5-6 are thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele to remove the lacZ sequence and neo selection cassette, leaving loxP sites flanking the critical exons 5-6. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml |
