| Primary Identifier | MGI:6490636 | Allele Type | Endonuclease-mediated |
| Gene | Pole3 | Strain of Origin | FVB |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | A 2 bp insertion (GC or CG) in the 3' end of the coding region was created using sgRNAs (targeting CGGGAGCAGAAAGGCAAG) and an ssODN template (CGGTTTGTCTAATTAAACCATAATATCCTGCTTCCTTACAGCGTACAGGCAGGAACAGAAGGGCAAGAAGGAGGCTTCGGAGCAAAAGAAGAAGGACAAAGACAAAAAGGA) with CRISPR/Cas9 technology. The resulting frameshift creates a large net positive charge on the normally negatively charged tail of the encoded peptide. |
