| Primary Identifier | MGI:6791321 | Allele Type | Targeted |
| Attribute String | Humanized sequence | Gene | Itgam |
| Transmission | Germline | Strain of Origin | C57BL/6J |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | A mutation was generated in exon 3 changing proline codon 77 (CCC) to histidine (CAC)(p.P77H). A loxP site was inserted into intron 2 and a second loxP site and an FRT site flanked neomycin resistance gene cassette into intron 3. The neo cassette was removed through subsequent flp-mediated recombination, leaving a conditional-ready allele with a floxed and mutated exon 3. The mutation mimics human SNP rs1143679 (p.R77H), which affects ligand binding of the encoded peptide. |
