| Primary Identifier | MGI:6779988 | Allele Type | Endonuclease-mediated |
| Gene | Adamts7 | Strain of Origin | C57BL/6J |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | CRISPR/cas9 genome editing is used to create an amino acid substitution at position 373 (glutamate to glutamine, GAG to CAG, E373Q) and a silent mutation in the Cas9 PAM site (Ala371A - GCC to GCT - to prevent additional editing). The mutation preserves the helical structure of the HExxH motif but prevents the generation of a reactive nucleophile at the zinc metalloprotease site. |
