| Primary Identifier | MGI:6790619 | Allele Type | Targeted |
| Attribute String | Epitope tag, Inducible, Recombinase | Gene | Tbx5 |
| Transmission | Germline | Strain of Origin | Not Specified |
| Induced With | tamoxifen | Is Recombinase | true |
| Is Wild Type | false |
| molecularNote | A cDNA for Cre/ERT2, followed by an IRES element, a Kozak consensus sequence, and a 2X FLAG epitope sequence was inserted in-frame into exon 2 of the gene, upstream of the endogenous translation start site. At 40bp downstream of exon 2, cloning sites (NotI and AflII) were added and a PGK-EM7-Neo-polyA cassette, flanked by Frt sites. The neo cassette was removed by crossing with Flpe expressing mice. |
