| Primary Identifier | MGI:6827291 | Allele Type | Endonuclease-mediated |
| Attribute String | Null/knockout | Gene | Ism1 |
| Strain of Origin | C57BL/6J | Is Recombinase | false |
| Is Wild Type | false |
| molecularNote | Introns 1 and 4 were targeted with sgRNAs and ssODN templates to insert loxP sites using CRISPR/Cas9 technology, resulting in the deletion of exons 2-4 after subsequent cre-mediated recombination. The loss of mRNA expression from this allele was confirmed by RT-qPCR. |
