| Primary Identifier | MGI:7256468 | Allele Type | Endonuclease-mediated |
| Attribute String | Humanized sequence | Gene | Epha2 |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | Using sgRNAs (targeting GTTCAGTGTACTTCAGTTGGNGG and TTCAGTGTACTTCAGTTGGTNGG) and an SSODN template (GGCCAGGTCCCGGTGCACGTAGTTCATGTTGGCCAGGTACTTCATGCCGGATGCGATACCCTGCAGCATGCCCACTAGCTGAAGTACACTGAACTCACCATCCTTCTCCTGCAGAGATAGGCCCTCAGTGCTGACCGG) with CRISPR/Cas9 technology, arginine codon 722 (AGG) in exon 13 was changed to glutamine (CAG) (p.R722Q). The equivalent p.R721Q human mutation is associated with age-related cortical cataract. |
