| Primary Identifier | MGI:7316773 | Allele Type | Endonuclease-mediated |
| Gene | Plk4 | Strain of Origin | (C57BL/6 x SJL)F2 |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | CRISPR/cas9 genome editing uses two guide RNAs (uORF1 sgRNA: 5'- CCGGCGGGAATTTTTCAAAA-3' and uORF2 sgRNA: 5'- GCGTAGAGAAGGCGTCCTGA-3') and a single-stranded DNA repair template to insert the desired modification (ATG start codons of uORF1 and uORF2 were changed to ATA). |
