| Primary Identifier | MGI:7341824 | Allele Type | Endonuclease-mediated |
| Attribute String | Conditional ready | Gene | Plk4 |
| Strain of Origin | (C57BL/6 x SJL/J)F1 | Is Recombinase | false |
| Is Wild Type | false |
| molecularNote | CRISPR/Cas9 genome editing uses guide RNAs, targeting DNA sequence 5'-aagctaggactttaatactc-3';, are used to insert a loxP flanked STOP cassette into intron 4 followed by a kinase-dead point mutation (D154A) into exon 5. Plk4-201 was used as reference for the exon number and the guide sequences. |
