Primary Identifier | MGI:7434982 | Allele Type | Endonuclease-mediated |
Attribute String | Null/knockout | Gene | Rpl3l |
Strain of Origin | C57BL/6NTac | Is Recombinase | false |
Is Wild Type | false |
molecularNote | CRISPR/Cas9 technology using sgRNAs 5â-GTGGGCCTTCTTCTGCCGAA-3â and 5â-TGGCTGAACACGCTGTGTAC-3âgenerated a frame-shift and subsequent deletion mutation in exon 5. Western blot analysis confirmed absence of protein in heart ventricle. |