| Primary Identifier | MGI:7439774 | Allele Type | Targeted |
| Attribute String | Modified isoform(s) | Gene | Comt |
| Transmission | Germline | Strain of Origin | (129S6/SvEvTac x C57BL/6NCrl)F1 |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | A construct containing a modified version of exon 2 with two point mutations (ATGCTG to GAGCTC) in the P2 promoter and a floxed pgk-neo cassette in intron 2 was inserted replacing the endogenous sequence via homologous recombination. One of the point mutations in exon 2 converts methionine to glutamic acid while the second is silent.Cre mediated recombination then removed the neo cassette. The mutant gene expresses only the soluble isoform, expression of the membrane-bound isoform is absent. |
