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Allele : Plk4<em2.1Ahol> polo like kinase 4; endonuclease-mediated mutation 2.1, Andrew Holland

Primary Identifier	MGI:7442584	Allele Type	Endonuclease-mediated
Attribute String	Null/knockout	Gene	Plk4
Strain of Origin	(C57BL/6J x SJL/J)F1	Is Recombinase	false
Is Wild Type	false

molecularNote

CRISPR/cas9 genome editing uses two sgRNAs RNAs (mPLK4 sgRNA1: 5'-aagctaggactttaatactc-3', mPLK4 sgRNA2: 5'-ctgcatgtagagggaagctg-3') and ssDNA to introduce two loxP sites into intron 4 and 5. Cre-mediated recombination removed exon 5.

mutations:
Insertion,
Intragenic deletion

earliestPublication:
Phan TP, et al. (2022) Upstream open reading frames control PLK4 translation and centriole duplication in primordial germ cells. Genes Dev 36(11-12):718-736

1 Feature

Genome

0 Expresses

0 Mutation Involves

Phenotype

Mouse alleles --> Mammalian phenotypes (MP terms)

Other

0 Carried By

0 Driven By

2 Publication categories

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