| Primary Identifier | MGI:7466201 | Allele Type | Endonuclease-mediated |
| Attribute String | Null/knockout, Recombinase | Gene | Aldh1a1 |
| Strain of Origin | C57BL/6J | Is Recombinase | true |
| Is Wild Type | false |
| molecularNote | Using CRISPR/Cas9 system, P2A-CRE-pA was inserted downstream of exon 10 of the Aldh1a1 gene. The premature termination of the transcript deleted the exons 11â13 corresponding to the 100 amino acids (401-500) of the C-terminal, which is essential for enzyme function and stability of Aldh1a1. It is a null allele. The absence of protein products was established by western blot analysis. |
