| Primary Identifier | MGI:7467072 | Allele Type | Endonuclease-mediated |
| Attribute String | Conditional ready, Reporter | Gene | Gt(ROSA)26Sor |
| Strain of Origin | Not Specified | Is Recombinase | false |
| Is Wild Type | false |
| molecularNote | The allele is generateb from Gt(ROSA)26Sorem1(CAG-EGFP/Rpl10)Mgmj by germline deletion of the lox-stop-lox cassette. Using CRISPR/Cas9 technology, a targeting vector CAG-FSF-LSL followed by an EGFP/Rpl10 fusion reporter gene under the control of the CAG promoter (CVM enhancer/chicken ACTB promoter) was inserted into the Gt(ROSA)26Sor locus. The lox-stop-lox cassette (LSL) was removed by crossing to a germ line cre-deleter. It is an Flp-dependent reporter. The expression of the EGFP/Rpl10 fusion reporter gene requires the removal of FSF by Flp recombinases. |
