Primary Identifier | MGI:7523854 | Allele Type | Endonuclease-mediated |
Attribute String | Inducible, Recombinase | Gene | Gt(ROSA)26Sor |
Strain of Origin | C57BL/6J | Induced With | blue light exposure |
Is Recombinase | true | Is Wild Type | false |
molecularNote | A 7.0 kb PA-Cre expression plasmid under the control of the CAG promoter was introduced at the Gt(ROSA)26Sor locus using the CRISPR/Cas9 system. PA-Cre consists of a split Cre (59/60) with each fragment complemented with nMag or pMag (respectively) and a nuclear localization sequence (NLS) (CreN59-nMag-NLS and NLS-pMag-CreC60), separated by P2A self-cleaving peptide sequence (2A). The nMag and pMag are part of Magnets protein derived from the fragments of photoreceptor gene product Vivid (VVD) from a filamentous fungi Neurospora crassa. |