| Primary Identifier | MGI:7506220 | Allele Type | Endonuclease-mediated |
| Attribute String | Epitope tag, Null/knockout, Reporter | Gene | Il18 |
| Strain of Origin | C57BL/6J | Is Recombinase | false |
| Is Wild Type | false |
| molecularNote | CRISPR/Cas9 genome editing technology was used to generate an insertion of a codon optimized 3x FLAG tag sequence followed by SV40-NLS sequence, an mNeongreen sequence followed by an SV40-NLS and 6-His tag sequences. After selection of guide RNAs as closest as possible to the IL-18 gene start and stop codons, all IL-18 sequences between start and stop codon (exons 2-6) were replaced by the mNeongreen cassette. |
