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Allele : Il18<em1Cga> interleukin 18; endonuclease-mediated mutation 1, Cem Gabay
Primary Identifier  MGI:7506220 Allele Type  Endonuclease-mediated
Attribute String  Epitope tag, Null/knockout, Reporter Gene  Il18
Strain of Origin  C57BL/6J Is Recombinase  false
Is Wild Type  false
molecularNote  CRISPR/Cas9 genome editing technology was used to generate an insertion of a codon optimized 3x FLAG tag sequence followed by SV40-NLS sequence, an mNeongreen sequence followed by an SV40-NLS and 6-His tag sequences. After selection of guide RNAs as closest as possible to the IL-18 gene start and stop codons, all IL-18 sequences between start and stop codon (exons 2-6) were replaced by the mNeongreen cassette.
  • mutations:
  • Insertion,
  • Intragenic deletion
  • synonyms:
  • IL-18 KO Neongreen reporter,
  • IL-18 KO Neongreen reporter
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1 Feature

Trail: Allele

Genome

0 Expresses

0 Mutation Involves

Phenotype

Mouse alleles --> Mammalian phenotypes (MP terms)

 

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Trail: Allele




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