| Primary Identifier | MGI:7518811 | Allele Type | Endonuclease-mediated |
| Attribute String | Conditional ready | Gene | Sirt1 |
| Strain of Origin | C57BL/6N | Is Recombinase | false |
| Is Wild Type | false |
| molecularNote | CRISPR/cas9 endonuclease-mediated genome editing is used to insert a loxP-flanked Sirt1 minigene that includes exons 5-9, the Sirt1 3' UTR and a STOP into intron 4 of the gene. In addition, a histidine to alanine amino acid substitution at codon 355 (H355A, CAT to GCT) is inserted into the endogenous exon 5. The mutation creates a catalytically dead allele. |
