| Primary Identifier | MGI:7607010 | Allele Type | Endonuclease-mediated |
| Attribute String | Humanized sequence, Inserted expressed sequence, Null/knockout | Gene | Fcgr1 |
| Strain of Origin | C57BL/6J | Is Recombinase | false |
| Is Wild Type | false |
| molecularNote | Using a CRISPR/Cas9 approach, an âactive genetic cassetteâ (AGC) was used to remove the endogenous mouse gene coding region and replace it with a functional human FCGR1A coding region under the control of the endogenous mouse promoter. The AGC included a Fcgr1 5âHR, Fcgr1 Exon1-Start-P2A sequence, human FCGR1A mini gene, hFCGR1A 3âUTR, U6 promoter, gRNA, Stop-FRT cassette, mFcgR1A Exon-1, mFcgR1A 3âHR. |
