| Primary Identifier | MGI:7580973 | Allele Type | Endonuclease-mediated |
| Attribute String | Null/knockout | Gene | Gsdma |
| Strain of Origin | Not Specified | Is Recombinase | false |
| Is Wild Type | false |
| description | This allele was generated in cis with Gsdma3tm1.1Lty allele. |
| molecularNote | CRISPR/Cas9 technology using 5â sgRNA 5â-CTCAACTGTGACTTTCCTGA-3â and 3â sgRNA 5'-TTTCTCAAGGGGAAAGACTG-3â was used to delete exons 2-11 in cis with the Gsdma3tm1.1Lty allele. Four founders (F1-9, F1-12, F1-16, and F1-18) were generated. F1-9 and F1-12 contain the same 11007 bp deletion, F1-16 has a 11024 bp deletion, and F1-18 has a 10998 bp deletion. This record represents pooled data from these lines or data where the line number is not specified. |
