| Primary Identifier | MGI:7712998 | Allele Type | Targeted |
| Attribute String | Inducible, Recombinase | Gene | Tnc |
| Transmission | Germline | Strain of Origin | (C57BL/6NTac x 129S6/SvEvTac)F1 |
| Induced With | tamoxifen | Is Recombinase | true |
| Is Wild Type | false |
| molecularNote | The targeting vector was designed to insert a Cre/ERT2 fusion protein into the tenascin C (Tnc) locus. Specifically, this vector includes (from 5' to 3') an FRT-flanked Neomycin-STOP, a cre/ERT2 fusion gene (Cre recombinase fused to a human estrogen receptor ligand binding domain) and rabbit beta-globin gene polyadenylation signal. The construct was electroporated into (C57BL/6NTac x 129S6/SvEvTac)F1-derived iTL BA1 embryonic stem (ES) cells and correctly targeted ES cells were injected into recipient blastocysts. The resulting chimeric mice were identified by genotyping and bred to a C57BL/6 FLP mouse line to remove the neomycin cassette. |
