| Primary Identifier | MGI:7712790 | Allele Type | Endonuclease-mediated |
| Attribute String | Epitope tag, Inserted expressed sequence | Gene | Nup107 |
| Strain of Origin | C57BL/6J | Is Recombinase | false |
| Is Wild Type | false |
| molecularNote | A sgRNA (gagcgagcgccgagaagacccgg) was designed to insert a HaloTag with a short linker and TEV (tobacco etch virus nuclear-inclusion-a endopeptidase) site immediately upstream of the start codon of the nucleoporin 107 (Nup107) gene. HaloTag is a 33 kDa haloalkane dehalogenase encoded by the DhaA gene from Rhodococcus rhodochrous that has been mutagenized to form an irreversible covalent bond to synthetic chloroalkane ligands. |