| Primary Identifier | MGI:7716884 | Allele Type | Endonuclease-mediated |
| Attribute String | Modified isoform(s) | Gene | Ncan |
| Strain of Origin | B6SJLF1/J | Is Recombinase | false |
| Is Wild Type | false |
| molecularNote | Exons 9-14 of the mouse Ncan gene were excised using CRISPR/cas9 methodologies. The mutation removes the EGF-like, Lectin-like, and Sushi domains (ELS), leaving the rest of the gene unaltered. It expresses an intact NCAN N-terminal domain but lacks the expression of the ELS domain by genomic PCR, immunofluorescence, and western blot |
