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Allele : Mis18a<em1(IMPC)Ics> MIS18 kinetochore protein A; endonuclease-mediated mutation 1, Mouse Clinical Institute

Primary Identifier  MGI:7781791 Allele Type  Endonuclease-mediated
Attribute String  Null/knockout Gene  Mis18a
Strain of Origin  C57BL/6N Is Recombinase  false
Is Wild Type  false
molecularNote  This allele was generated at the Institut Clinique de la Souris by electroporating Cas9 protein and 4 sgRNAs (equivalent to TGGGAAGTGGGGTACTCCCG, TCCCGTGGTTCAGCACTAAA,TGGTGATAGCCCCTTAAGAT and AGCCCCTTAAGATCGGAAAG), resulting in a 548 bp deletion that includes exon 2 (ENSMUSE00001224879; GRCm39), leading to a frameshift and premature stop codon.
  • mutations:
  • Intragenic deletion
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1 Feature

Genome

0 Expresses

0 Mutation Involves

Phenotype

Mouse alleles --> Mammalian phenotypes (MP terms)

 

Other

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0 Driven By

2 Publication categories