| Primary Identifier | MGI:7738768 | Allele Type | Endonuclease-mediated |
| Attribute String | Null/knockout | Gene | Pbx4 |
| Strain of Origin | (C57BL/6J x DBA)F1 | Is Recombinase | false |
| Is Wild Type | false |
| molecularNote | CRISPR/Cas9 genome editing technology was used to generate a 19bp deletion in the genomic region corresponding to the PBC domain in exon 4. The 19-bp deletion is located in the predicted coding region of mRNA transcript 1 but not in the shorter transcript 2 and is expected to result in a frameshift and premature termination of translation. Western blot analysis confirmed the absence of protein expression in testicular lysates from homozygous mutant males. |
