| Primary Identifier | MGI:7856624 | Allele Type | Endonuclease-mediated |
| Attribute String | Not Specified | Gene | Folh1 |
| Strain of Origin | C57BL/6NCrl | Is Recombinase | false |
| Is Wild Type | false |
| molecularNote | TALENs were designed to target exon 11 of murine gene as follows: the left TALEN possessed the RVD sequence NG NG NN HD NI NI NN HD NG NN NN NN NI NG NN (targeted DNA sequence 5'-TTGCAAGCTGGGATG-3'), the right TALEN possessed the RVD sequence HD NI NN NG NI NN NI NI HD HD NI NI NN NI NI (targeted DNA sequence 5'-TTCTTGGTTCTACTG-3'). The TALENs were targeted to cleave within the chromosomal DNA sequence 5'CAGAAGAATTTGGCC-3'. One founder mouse carrying deletion of 6 bp (5'-CAGAAGGCC-3') was chosen to establish this allele. The deletion lies in the region encoding the active site of the protein. |
