Primary Identifier | MGI:3027823 | Allele Type | Targeted |
Attribute String | Null/knockout | Gene | Spo11 |
Transmission | Germline | Strain of Origin | 129S6/SvEvTac |
Is Recombinase | false | Is Wild Type | false |
molecularNote | Exon 1 starting after the sequence coding for the 3rd amino acid after the second "in frame" ATG through nucleotide 35 of the first intron was replaced with a PGK-neomycin cassette in reverse orientation. This removes the splice donor site. Absence of transcript was confirmed by Northern blot analysis. |