Primary Identifier | MGI:3038949 | Allele Type | Targeted |
Attribute String | Null/knockout, Reporter | Gene | Btg2 |
Transmission | Germline | Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl<+> |
Is Recombinase | false | Is Wild Type | false |
molecularNote | Homologous recombination was used to replace the coding portion of exon 1 with GFPpA fused to a floxed neo cassette. Subsequent cre-mediated recombination removed the neo cassette, leaving GFPpA and one loxp site. The level of GFP fluorescence in developing CNS of heterozygous mice E9.5-E14.5 matched known expression of the endogenous protein. |