| Primary Identifier | MGI:5585618 | Allele Type | Targeted |
| Gene | a | Inheritance Mode | Dominant |
| Transmission | Germline | Strain of Origin | C57BL/6NTac |
| Is Recombinase | false | Is Wild Type | false |
| description | Germline deletion of the frt-flanked selection cassette was achieved by crossing mice bearing the selection cassette (in place of the retrotransposon insertion) to C57BL/6NTac-Tg(CAG-flpe)2Arte "flp-deleter" mice. (J:213411) |
| molecularNote | The 14.7 kb retrotransposon insertion in intron 1 of the a (nonagouti) allele, which functionally inactivates the gene, was replaced by a neomycin and puromycin resistance cassette flanked by frt sequences. The selection cassette was excised by flp recombinase, leaving a single frt site in the intron. |
