Primary Identifier | MGI:6716018 | Allele Type | Targeted |
Attribute String | Reporter | Gene | Il11 |
Transmission | Germline | Strain of Origin | C57BL/6 |
Is Recombinase | false | Is Wild Type | false |
molecularNote | An EGFP gene with poly(A) signal sequence was inserted between the 2nd and 3rd nucleotide of exon 2 of the Il11 gene on BAC clone RP23-285B12 to create an Il11 promoter-driven Il11/EGFP fusion gene coding for the first three Il11 amino-acids followed by the EGFP peptide. The modified BAC was injected into oocytes together with sperm through Intracytoplasmic sperm injection (ICSI). |